番石榴4個品種葉和幼果的生物活性和酚類成分研究

基金項目:

福建省自然科學(xué)基金項目（2020J011369）；福建省科技計劃公益類專項（2019R1030-4）資助

Biological Activities and Phenolic Constituents in Extracts from Leaves and Young Fruits of Four Guava Cultivars

Author:

WU Miaohong

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
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QIU Shanlian

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
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LIN Baomei

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
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ZHANG Shuai

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
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HONG Jiamin

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
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ZHENG Kaibin

Institute of Technology Services, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
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摘要:

為提高番石榴（Psidium guajava）副產(chǎn)物的綜合利用率，測定了‘珍珠’、‘水蜜’、‘紅寶石’和‘西瓜’4個品種葉和幼果提取物中的酚類與黃酮含量，并分析了其與清除DPPH·、ABTS+、HO·自由基能力和α-葡萄糖苷酶抑制活性的相關(guān)性，并用超高效液相色譜-串聯(lián)質(zhì)譜法（UPLC-MS/MS）分析了提取物的酚類成分。結(jié)果表明，4個番石榴品種葉的酚類和黃酮含量、清除自由基能力、α-葡萄糖苷酶抑制活性均顯著高于幼果，‘珍珠’葉的酚類含量最高，生物活性最強。酚類、黃酮含量與清除自由基能力呈極顯著正相關(guān)（P<0.01），與α-葡萄糖苷酶抑制活性呈顯著正相關(guān)（P<0.05）。UPLC-MS/MS鑒定結(jié)果表明，4個品種葉酚類含量較高的均為槲皮素-3-O-葡萄糖苷、金絲桃苷、鞣花酸和楊梅苷，除鞣花酸外大部分存在于葉的酚類成分也存在于幼果中，且含量顯著高于幼果。因此，4個品種中，‘珍珠’葉是開發(fā)天然抗氧化劑和α-葡萄糖苷酶抑制劑的優(yōu)良資源。

Abstract:

In order to improve the comprehensive utilization rate of the by-products of guava (Psidium guajava), the biological activities and phenolic constituents of extracts from 4 cultivars (‘Zhenzhu’, ‘Shuimi’, ‘Hongbaoshi’, ‘Xigua’) of guava were studied. The contents of total phenols and flavonoids were determined, then the correlations between the contents of phenols and flavonoids with biological activities were analyzed, including the scavenging abilities on DPPH·, ABTS+, HO· free radicals, and the α-glucosidase inhibitory activity. Furthermore, the phenolic constituents of extracts were identified by ultra-high performance liquid chromatography and the triple-quadrupole tandem mass spectrometry (UPLC-MS/MS). The results showed that the contents of total phenols and flavonoids, the free radicals scavenging abilities and α-glucosidase inhibitory activity in leaves of 4 cultivars were significantly higher than those in young fruits, ‘Zhenzhu’ leaf had the highest content of total phenols as well as the most potent free radicals scavenging abilities and α-glucosidase inhibitory activity. Correlation analysis revealed that the contents of phenols and flavonoids were significantly positively correlated with the scavenging abilities towards DPPH·, ABTS+, HO· free radicals (P<0.01), as well as the α-glucosidase inhibitory activity (P<0.05). UPLC-MS/MS analysis showed that the contents of quercetin-3-O-glucoside, hyperoside, ellagic acid and myricitrin in leaves of 4 cultivars were high, and most of the constituents found in leaves except ellagic acid also existed in the young fruits, and the contents in leaves were higher than those in young fruits. Therefore, ‘Zhenzhu’ guava leaf could be a good resource for developing natural antioxidant and α-glucosidase inhibitor among 4 cultivars.

吳妙鴻,邱珊蓮,林寶妹,張帥,洪佳敏,鄭開斌.番石榴4個品種葉和幼果的生物活性和酚類成分研究[J].熱帶亞熱帶植物學(xué)報,2021,29(6):694~702

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