XU Hai-tang, LIAO Hua-zhen, ZHAO Yan-zhi, ZHANG Jin-yan, ZHOU Ju-ying. Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components[J]. Science and Technology of Food Industry, 2019, 40(22): 157-162. DOI: 10.13386/j.issn1002-0306.2019.22.028

Citation: XU Hai-tang, LIAO Hua-zhen, ZHAO Yan-zhi, ZHANG Jin-yan, ZHOU Ju-ying. Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components[J]. Science and Technology of Food Industry, 2019, 40(22): 157-162. DOI: 10.13386/j.issn1002-0306.2019.22.028

響應(yīng)面法優(yōu)化山豆根多糖提取工藝及其分級醇沉組分的抗氧化活性

許海棠,  廖華珍1,  趙彥芝1,  張金彥1,  周菊英1, , 

1. 廣西民族大學化學化工學院, 廣西林產(chǎn)化學與工程重點實驗室, 廣西南寧 530008;

2. 廣西民族大學海洋與生物技術(shù)學院, 廣西海洋微生物資源產(chǎn)業(yè)化工程技術(shù)研究中心, 廣西南寧 530008

詳細信息

作者簡介:

許海棠(1975-),女,碩士,高級實驗師,研究方向:天然藥物資源的開發(fā)研究,E-mail:xhthellen@163.com。

通訊作者:

周菊英(1979-),女,博士,教授,研究方向:天然產(chǎn)物資源的研究與開發(fā),E-mail:zhoujuying@126.com。

中圖分類號: TS255.1

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Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components

1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China;

2. Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China

摘要

摘要: 為研究山豆根多糖的提取工藝及其抗氧化性,采用熱水浸提法提取山豆根粗多糖(SGP),研究提取溫度、提取時間、液料比對多糖得率的影響,在單因素實驗基礎(chǔ)上采用響應(yīng)面法對山豆根粗多糖的提取工藝進行條件優(yōu)化。將提取得到的粗多糖分級醇沉,并分別采用清除DPPH、ABTS+自由基及還原能力的方法對各醇沉組分多糖的抗氧化活性進行評估。結(jié)果表明,山豆根粗多糖的最佳提取工藝條件為:提取溫度83℃,提取時間133 min,液料比30:1 mL/g。在此工藝條件下,山豆根粗多糖得率為3.98%。粗多糖經(jīng)分級醇沉共獲得SGP50、SGP70、SGP80和SGP90 4個組分,且SGP90表現(xiàn)出最強的抗氧化能力,尤其是在清除DPPH自由基方面,顯著高于其它組分(P<0.05)。

Abstract: To explore the extraction process and antioxidant activity of polysaccharide from Sophora tonkinensis Gagnep,the Sophora tonkinensis Gagnep was used for extracting polysaccharide with hot water. The effects of extraction temperature,extraction time and ratio of liquid to material on the extraction rate of polysaccharide were investigated. On the basis of single factor tests,the extraction technology of polysaccharide from Sophora tonkinensis Gagnep was optimized by response surface methodology(RSM). The extracted crude polysaccharide was precipitated and fractionated by ethanol,and the antioxidant activity of each alcohol component was evaluated by the methods of scavenging DPPH,ABTS+ radical and reducing ability,respectively. Results showed that,the optimum extraction conditions were as follows:The extraction temperature was 83 ℃,the extraction time was 133 min,the ratio of liquid to material was 30:1 mL/g. Under the optimal extraction conditions,the yield of polysaccharides from Sophora tonkinensis Gagnep was 3.98%. The crude polysaccharide obtained four components SGP50,SGP70,SGP80 and SGP90 by fractional alcohol precipitation. And the SGP90 showed the strongest antioxidant activity,especially in the DPPH free radical scavenging,which was significantly higher than other components(P<0.05).

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計量 文章訪問數(shù):  HTML全文瀏覽量:  PDF下載量:  出版歷程 收稿日期:  2019-02-21 網(wǎng)絡(luò)出版日期:  2020-11-12 刊出日期:  2019-11-14

目錄

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