GONG Xin-huai, LI Ming-chun, XIN Mei-hua, ZHAO Xiao-jie, LV Gan, XU Jie, ZHAO Sheng-yun. Optimization of the Extraction Technology of Tea Polyphenols from Tea Waste by Aqueous Two-phase Systems with Ionic Liquids as Additives[J]. Science and Technology of Food Industry, 2020, 41(15): 158-166. DOI: 10.13386/j.issn1002-0306.2020.15.025

Citation: GONG Xin-huai, LI Ming-chun, XIN Mei-hua, ZHAO Xiao-jie, LV Gan, XU Jie, ZHAO Sheng-yun. Optimization of the Extraction Technology of Tea Polyphenols from Tea Waste by Aqueous Two-phase Systems with Ionic Liquids as Additives[J]. Science and Technology of Food Industry, 2020, 41(15): 158-166. DOI: 10.13386/j.issn1002-0306.2020.15.025

離子液體輔助雙水相系統(tǒng)提取茶渣中茶多酚工藝優(yōu)化

1. 武夷學(xué)院生態(tài)與資源工程學(xué)院, 福建省生態(tài)產(chǎn)業(yè)綠色技術(shù)重點(diǎn)實(shí)驗(yàn)室, 福建武夷山 354300;

2. 華僑大學(xué)材料科學(xué)與工程學(xué)院, 環(huán)境友好功能材料教育部工程研究中心, 福建廈門 361021

詳細(xì)信息

作者簡(jiǎn)介:

龔新懷(1985-),男,博士,副教授,主要從事生物質(zhì)高值化利用與復(fù)合材料方面的研究,E-mail:wyu_gxh@163.com。

中圖分類號(hào): TS255.1

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Optimization of the Extraction Technology of Tea Polyphenols from Tea Waste by Aqueous Two-phase Systems with Ionic Liquids as Additives

1. Ecology and Resources Engineering College, Wuyi University, Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, Wuyishan 354300, China;

2. College of Material Science and Engineering, Huaqiao University, Engineering Research Center of Environment-Friendly Functional Materials, Ministry of Education, Xiamen 361021, China

摘要

摘要: 為發(fā)展一種綠色、高效及溫和的茶多酚提取方法,采用離子液體(IL)輔助乙醇/硫酸銨雙水相系統(tǒng)(ATPS)提取茶渣中的茶多酚,考察了離子液體結(jié)構(gòu)和用量對(duì)茶多酚得率和抗氧化性的影響,對(duì)提取工藝進(jìn)行了正交試驗(yàn)優(yōu)化。結(jié)果顯示,少量IL添加可提高乙醇/硫酸銨ATPS的分相能力及其對(duì)茶多酚的得率,但對(duì)提取液的抗氧化活性沒(méi)有明顯影響;咪唑型離子液體對(duì)提取茶多酚具有更好的促進(jìn)作用,咪唑環(huán)上側(cè)鏈越長(zhǎng),其茶多酚得率越高;[C4mim]Cl輔助乙醇/硫酸銨ATPS對(duì)茶渣中茶多酚得率明顯高于其他體系,且其提取液中兒茶素類組分含量更高。茶渣多酚最佳提取工藝為[C4mim]Cl質(zhì)量分?jǐn)?shù)10%、硫酸銨質(zhì)量分?jǐn)?shù)30%、乙醇體積分?jǐn)?shù)60%,料液比1∶40 g/mL及超聲功率540 W,在此條件下,茶渣中茶多酚得率為85.31±1.25 mg·g-1。IL輔助雙水相系統(tǒng)是一種有前景的茶多酚提取工藝,在保持茶多酚活性的同時(shí),能獲得較高的茶多酚得率和兒茶素類組分含量。

Abstract: In order to propose a green, efficient and gentle method for extraction of tea polyphenols, ionic liquids (ILs) assisted ethanol/ammonium sulphate aqueous two phase system (C2H5OH/(NH4) 2SO4 ATPS) was used as a alternative technique for extraction of the tea polyphenols in tea biomass, the effects of ionic liquids structures and amounts on the yield and antioxidant activity of tea polyphenols were studied, the optimal extraction was explored by orthogonal test. The results showed that the C2H5OH/(NH4) 2SO4 ATPS added with ILs as adjuvants in small amounts displayed a better phase separation and a higher yield, but no notable influence on the antioxidant activity of the extracts. The ILs with imidazolium cation showed a better improvement in extraction of tea polyphenols, and higher yield of tea polyphenols was obtained when the imidazolium ionic liquids with a longer side chain. The[C4mim]Cl assisted C2H5OH/(NH4) 2SO4 ATPS showed a higher yield of polyphenols and a higher content of catechin than other extraction systems. The optimal extraction conditions of tea polyphenols concluded by the corresponding surface optimization method was that:[C4mim]Cl and (NH4) 2SO4 mass fraction was 10% and 30%, ethanol volume fraction was 60%, liquid to solid ratio was 1:40 g/mL, ultrasonic power was 540 W, thus the yield of tea polyphenols obtained was 85.31±1.25 mg·g-1 at the conditions. Ionic liquids assisted ATPS showed an promising extraction of polyphenols, while the biological activities of the tea polyphenols were remained and a higher yield of polyphenols and content of catechin components were obtained.

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目錄

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