HUANG Ye-chuan, LI Feng, YAN Cheng. Effect of lipoxygenase on the intramuscular lipid oxidation in pork due to high pressure treatment[J]. Science and Technology of Food Industry, 2015, (14): 147-151. DOI: 10.13386/j.issn1002-0306.2015.14.022

Citation: HUANG Ye-chuan, LI Feng, YAN Cheng. Effect of lipoxygenase on the intramuscular lipid oxidation in pork due to high pressure treatment[J]. Science and Technology of Food Industry, 2015, (14): 147-151. DOI: 10.13386/j.issn1002-0306.2015.14.022

高壓促進(jìn)豬肉肌內(nèi)脂肪氧化過程中脂肪氧化酶的作用

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作者簡介:

黃業(yè)傳(1975-),男,博士,副教授,研究方向:肉制品食品加工與酶工程。;

中圖分類號: TS251.51

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Effect of lipoxygenase on the intramuscular lipid oxidation in pork due to high pressure treatment

College of Life Science and Engineering,Southwest University of Science and Technology

摘要

摘要: 為研究高壓促進(jìn)豬肉肌內(nèi)脂肪氧化過程中脂肪氧化酶(Lipoxygenase,LOX)的作用,以去除LOX的豬背最長肌為原料,在其中加入事先從豬肉中提取的或外源LOX(大豆脂肪氧化酶),經(jīng)600MPa、50℃或350MPa、20℃處理并經(jīng)6d冷藏后,測定各樣品高壓處理和冷藏后LOX活性和TBARS值。結(jié)果表明:LOX對高壓下肌內(nèi)脂肪氧化的啟動(dòng)有重要作用,但其不會(huì)影響樣品冷藏后的最終氧化狀態(tài)(TBARS值);在高壓處理后的冷藏中,LOX的作用不明顯,主要以脂肪自動(dòng)氧化為主,即使加入5倍濃度的外源LOX,也只引起樣品最終TBARS值的少量增加。因此,高壓促進(jìn)豬肉肌內(nèi)脂肪氧化中主要以自動(dòng)氧化為主,而LOX的作用很小。 

關(guān)鍵詞: 高壓  /  豬肉  /  脂肪氧化  /  脂肪氧化酶  

Abstract: To investigate the effect of lipoxygenase(LOX) on the intramuscular lipid oxidation in pork due to high pressure treatment,porcine longissimus muscle which had been removed of LOX was used as material,after addition of some LOX extracted from fresh porcine longissimus muscle or exogenous LOX(from soybean),the samples were treated at 600MPa-50℃ or 350MPa-20℃ before 6 days storage at 4℃,then the activities of LOX and TBARS values in all samples after high pressure treatment and after cold storage were analyzed. The results showed that LOX had important role on the start-up of intramuscular lipid oxidation due to high pressure treatment,but it had no effect on the last oxidation state(TBARS value) of chilled samples. During cold storage after high pressure treatment,the lipid oxidation was mainly the auto-oxidation,and the role of LOX was very limited,even 5 times of exogenous LOX was added,the TBARS value of the chilled samples only increased a little. So the role of LOX on the intramuscular lipid oxidation in pork due to high pressure treatment was very limited compared to auto-oxidation.

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