摘要: 目的:從綠茶茶渣中分離半纖維素,研究其組成性質(zhì)。方法:利用堿性過氧化氫法浸提、乙醇沉淀提取得到茶渣半纖維素。紅外光譜法測定其糖苷鍵類型;離子色譜法分析其單糖組成;高效凝膠色譜法測定其相對分子質(zhì)量。結(jié)果:紅外光譜中半纖維素具有糖類物質(zhì)的特征吸收峰;茶渣半纖維素主要由葡萄糖、半乳糖、阿拉伯糖組成,另外還含有少量的鼠李糖、木糖和氨基半乳糖。高濃度乙醇沉淀的茶渣半纖維素相對分子量在2000左右;結(jié)論:該茶渣半纖維素是一種酸性半纖維素多糖。 

Abstract: Objective:To get hemicelluloses polysaccharide from green tea-leaf and study the composition of the polysaccharide. Methods:The hemicelluloses was obtained from tea-leaf that without most water soluble composition by ways as follows:Alkaline hydrogen peroxide extraction, graded ethanol precipitation. The form of glycosidic bond was determined by IR. The monosaccharide components of hemicelluloses were determined by Ion Chromatograph. Its MW was measured by HPGFC. Results:Typical absorption of sugar was shown in the IR spectrum. Glucose, galactose, arabinose, rhamnose, mannose and galactosamine. The MW of the hemicelluloses which were precipitated by high concentrations of ethanol was estimated 2000. Conclusion:The hemicelluloses extract by alkaline hydrogen peroxide was a kind of acidic hemicelluloses polysaccharide.

[1] 王黎明.具有降血糖活性的茶多糖組分分離純化和結(jié)構(gòu)鑒定[D].無錫:江南大學(xué), 2006. [2]

Revanappa S B, Salimath P V.Structure characterization of hemicelluloses A from whea (tTriticum aestivum) vatieties differing in their chapatti-making quality[J].Carbohydrate Polymers, 2010, 79:655-659.

[3]

Pai P, Feng P, Jing B, et al.Isolation and structural characterization of hemicelluloses from bamboo species Phyllostachys incarnate Wen[J].Carbohydrate Polymers, 2011, 86:883-890.

[4]

Feng P, Jun-Li R, Feng X, et al.Fractional study of alkali-soluble hemicelluloses obtained by graded ethanol precipitation from sugar cane bagasse[J].Journal of Agricultural and Food Chmeistry, 2010, 58:1768-1776.

[5]

Yong-Chang S, Jia-Long W, Feng X, et al.Organosolv-and alkali-soluble hemincelluloses degraded from Tamarix austromongolica:Characterization of physicochemical, structural features and thermal stability[J].Polymer Degradation and Stability, 2011, 96:1478-1488.

[6]

Egues I, Sanchez C, Mondragon I, et al.Effect of alkaline and autohydrolysis processes on purity of obtained hemicelluloses from corn stalks[J].Bioresource Technology, 2012, 103:239-248.

[7]

Ebingerova A, Hromadkova Z, Heinze T.Hemicellulose[J].Advances in Polymer Science, 2005, 186:1-67.

[8] 史德芳, 楊洋, 任紅.仙人掌多糖提取過程中三種脫蛋白方法的比較研究[J].山東食品發(fā)酵, 2006 (2) :42-44. [9]

Zhou X L, Sun P N, Bucheli P, et al.FT-IR methodology for quality control of arabinogalactan protein (AGP) extracted from green tea (Camellia sinensis) [J].Journal of Agricultural and Food Chemisty, 2009, 57:5121-5128.

[10] 戴軍, 朱松, 湯堅.PMP柱前衍生高效液相色譜法分析杜氏鹽藻多糖組成[J].分析測試學(xué)報, 2007 (2) :206-210. [11] 徐燕, 朱科學(xué), 錢海峰, 等.桃膠多糖的提取分離機(jī)組成性質(zhì)研究[J].食品工業(yè)科技, 2008 (1) :66-71. [12] 張惟杰.糖復(fù)合物生化研究技術(shù)[M].杭州:浙江大學(xué)出版社, 1999:10-11. [13] 張云貴, 劉祥云, 李天俊, 等.生物化學(xué)實驗指導(dǎo)[M].北京:中國農(nóng)業(yè)出版社, 2007-12. [14] 中華人民共和國農(nóng)牧漁業(yè)部.NY82.11-1988果膠的測定[S].北京:中國標(biāo)準(zhǔn)出版社, 1988. [15] 彭鋒.農(nóng)林生物質(zhì)半纖維素分離純化、結(jié)構(gòu)表征及化學(xué)改性的研究[D].廣州:華南理工大學(xué), 2010. [16]

Fang J M, Sun R C, Salisbury D, et al.Comparative study of hemicelluloses from wheat straw by alkali and hydrogen peroxide extractions[J].Polymer Degradation and Stability, 1999, 66:423-432.

[17] 蔚慰奇, 武書彬, 彭云云.麥草水溶性和堿溶性半纖維素的分離和表征[J].林產(chǎn)化學(xué)與工業(yè), 2010 (6) :66-70.

相關(guān)知識

綠茶素
茶葉渣也是寶 茶葉渣的十大妙用
孕婦怎么攝取膳食纖維
蘋果+綠茶＝只要1元的減肥法
茶葉枕可幫助提升睡眠質(zhì)量
復(fù)旦大學(xué)研究：茶葉是打通人體經(jīng)絡(luò)最有效的食品？
運(yùn)動前喝茶，燃脂提升17%
紅茶VS綠茶 誰更適合減肥？
女性經(jīng)期可以喝綠茶嗎？
夏天喝綠茶的14個好處

網(wǎng)址: 綠茶茶渣半纖維素的提取及組成性質(zhì)研究 http://m.u1s5d6.cn/newsview261478.html