摘要

高血糖會導(dǎo)致線粒體氧化應(yīng)激增加，細(xì)胞內(nèi)炎癥信號異常激活，并介導(dǎo)多種功能障礙。覆盆子酮（RK）是一種芳香族酚類化合物，存在于許多植物中，可幫助減肥、恢復(fù)受損的葡萄糖耐量，并具有抗氧化特性。在我們的研究中，覆盆子酮可大大預(yù)防鏈脲佐菌素（STZ）誘導(dǎo)的高血糖斑馬魚模型中胰島、大腦和其他組織因高血糖造成的損傷。RK 還能恢復(fù)體重、胰島素水平和食物攝入量指標(biāo)。通過轉(zhuǎn)錄組分析，我們發(fā)現(xiàn)服用 RK 能顯著減少 STZ 誘導(dǎo)的胰島素合成和胰腺分泌，并能改變蛋白質(zhì)和碳水化合物的代謝。代謝組學(xué)分析結(jié)果顯示，RK還能預(yù)防STZ誘導(dǎo)的代謝紊亂，如腺苷和鞘脂代謝。代謝組和轉(zhuǎn)錄組數(shù)據(jù)的整合分析以及關(guān)鍵代謝調(diào)控基因（glut1、glut2、ctrb1、cka、ck、pklr）的qRT-PCR驗(yàn)證證實(shí)，嘌呤通路是富集程度最高的代謝通路，其中代謝物的積累和基因表達(dá)水平在RK處理后呈現(xiàn)一致的變化規(guī)律。我們的研究為了解 RK 的降血糖機(jī)制提供了一個新的視角，可能有助于利用斑馬魚高血糖模型研究降血糖藥物的作用模式。

本文章由計算機(jī)程序翻譯，如有差異，請以英文原文為準(zhǔn)。

Study on the signaling pathways involved in the anti-hyperglycemic effect of raspberry ketone on zebrafish using integrative transcriptome and metabolome analyses?

Hyperglycemia leads to increased oxidative stress in mitochondria, an abnormal activation of intracellular inflammatory signals, and mediate multiple dysfunctions. Raspberry ketone (RK) is an aromatic phenolic compound found in many plants and could contribute to weight loss, restore impaired glucose tolerance, and has antioxidant properties. In our investigation, RK could greatly prevent islet, brain and other tissue damage caused by hyperglycemia in a zebrafish model with streptozotocin (STZ)-induced hyperglycemia. Body weight, insulin level, and food intake indexes were also restored by RK. Using transcriptome profiling, we found that RK administration could significantly attenuate STZ-induced insulin synthesis and pancreatic secretion as well as alter protein and carbohydrate metabolism. Metabolomics analysis results showed that RK could also prevent STZ-induced metabolic disorders, such as adenosine and sphingolipid metabolism. Integrative analysis of metabolome and transcriptome data and qRT-PCR validation of key metabolic regulatory genes (glut1, glut2, ctrb1, ccka, gck, pklr) confirmed that the purine pathway was the most enriched metabolic pathway, in which both metabolite accumulation and gene expression levels showed consistent change patterns upon RK treatment. Our study provides a new perspective for understanding the hypoglycemic mechanism of RK and may be helpful for investigating the modes of action of hypoglycemic drugs using the zebrafish hyperglycemia model.

相關(guān)知識

覆盆子酮對糖尿病模型小鼠的降血糖作用及其機(jī)制研究
覆盆子酮葡萄糖甙的美白功效
覆盆子酮（樹莓酮）
覆盆子酮在健康領(lǐng)域的多元功效探析
覆盆子酮合成的動力學(xué)及工藝的分析研究.pdf
覆盆子酮的合成研究.pdf
覆盆子酮的化學(xué)合成研究進(jìn)展
覆盆子酮功效及副作用(覆盆子酮：功效與副作用一覽)
覆盆子酮功效及副作用
覆盆子酮的合成研究(精選).doc

網(wǎng)址: 利用轉(zhuǎn)錄組和代謝組的綜合分析，研究覆盆子酮對斑馬魚的降血糖作用所涉及的 http://m.u1s5d6.cn/newsview1309574.html